Investigation of product derived lymphoma following infusion of piggyBac modified CD19 chimeric antigen receptor T-cells
We carried out a Part I scientific trial of donor derived CD19-specific chimeric antigen receptor T-cells (CAR T-cells) for B-cell malignancy that relapsed or endured after matched associated allogeneic hemopoietic stem cell transplant. To beat the associated fee and transgene capability limitations of conventional viral vectors, CAR T-cells have been produced utilizing the piggyBac transposon system of genetic modification. Following CAR T-cell infusion, one affected person developed a progressively enlarging retroperitoneal tumor on account of a CAR expressing CD4+ T-cell lymphoma. Screening of different sufferers led to the detection of a second CAR T-cell tumor in thoracic para-aortic lymph nodes in an asymptomatic affected person.
Evaluation of the primary lymphoma confirmed a excessive transgene copy quantity, however no insertion into typical oncogenes. There have been additionally structural modifications comparable to altered genomic copy quantity and level mutations unrelated to the insertion websites. Transcriptome evaluation confirmed transgene promoter pushed upregulation of transcription of surrounding areas regardless of insulator sequences surrounding the transgene. Nevertheless, marked international modifications in transcription predominantly correlated with gene copy quantity slightly than insertion websites.
In each sufferers, the CAR T-cell derived lymphoma progressed and one affected person died. We describe the primary two instances of malignant lymphoma derived from CAR gene modified T-cells. Though CAR T-cells have an enviable report of security to this point, our outcomes emphasize the necessity for warning and common comply with up of CAR T recipients, particularly when novel strategies of gene switch are used to create genetically modified immune therapies. The trial was registered at www.anzctr.org.au as ACTRN12617001579381.
Reminiscence stem T cells modified with a redesigned CD30-chimeric antigen receptor present an enhanced antitumor impact in Hodgkin lymphoma
Goals: Adoptive cell remedy (ACT) with mature T cells modified with a chimeric antigen receptor has demonstrated improved consequence for B-cell malignancies. Nevertheless, its utility for others comparable to Hodgkin lymphoma stays a scientific problem. CD30 antigen, expressed in Hodgkin lymphoma cells, is absent in most wholesome tissues, representing a perfect goal of ACT for this illness. Regardless of that, efficacy of CD30-chimeric antigen receptor (CAR) T cells for Hodgkin lymphoma stays modest. Right here, now we have developed and examined a novel CD30-CAR T to enhance efficacy of CD30-CAR remedy, utilizing a concentrating on epitope inside the non-cleavable a part of CD30 receptor, and reminiscence stem T cells (TSCM) to enhance engraftment, persistence and antitumor exercise.
Strategies: TSCM-like cultures have been generated and expanded ex vivo and transduced at day 1 or 2 with a lentiviral vector encoding the CD30-CAR. Therapeutic in vivo experiments have been carried out utilizing NSG mice injected with L540 (sc) or L428 (iv) and handled with CD30-CAR T cells when the tumor was established.
Outcomes: CD30-CAR TSCM-like cells generated and expanded ex vivo, regardless of CD30 expression and fratricide killing of CD30+ CAR T cells, weren’t impaired by soluble CD30 and utterly eradicated Hodgkin lymphoma in vivo, displaying excessive persistence and long-lasting immunity. As well as, extremely enriched CD30-CAR TSCM-like merchandise confer a survival benefit in vivo, in distinction to extra differentiated CAR T cells, with greater tumor infiltration and enhanced antitumor impact.
Conclusion: This research helps using a refined CD30-CAR T cells with extremely enriched TSCM-like merchandise to enhance scientific efficacy of CAR T for Hodgkin lymphoma.
Chimeric antigen receptor T cells concentrating on CD7 in a toddler with high-risk T-cell acute lymphoblastic leukemia
Efficient systemic therapies for relapsed or refractory T-cell acute lymphoblastic leukemia (T-ALL) are restricted. Current scientific utility of chimeric antigen receptor (CAR) immunotherapy has demonstrated profitable management of B-cell malignancies by CAR-T cells; nonetheless, designing CARs for T-ALL stays a problem. CD7 overexpression in T-cell malignancies could also be a horny goal for immunotherapy in T-ALL. This research aimed to explain the protected and efficient use of autologous CD7-CAR T cells (4SCAR7) for the therapy of T-ALL with induction failure in an 11-year-old affected person. Based mostly on The Chinese language Youngsters’s Most cancers Group-ALL (CCCG-ALL) research protocol, minimal residual illness (MRD) by move cytometry (FC) evaluation was detected on days 19 and 46 of remission induction.
On the finish of remission-induction chemotherapy, the affected person achieved morphologic full remission, although with MRD 16.13% and RT-PCR of KMT2A-MLLT1 fusion constructive, which indicated induction failure. The cerebrospinal fluid (CSF) was damaging for blasts at identified. CAR-T remedy and allogeneic transplant have been really helpful as the subsequent therapy choices. CD3+ lymphocytes have been collected from the affected person 18 days after the high-dose MTX chemotherapy by means of leukapheresis. The 4SCAR7 CD7-targeting CAR-T cells have been generated thereafter.
The affected person acquired lymphodepleting chemotherapy previous to 4SCAR7 infusion. Oral administration of itraconazole and sulfamethoxazole was carried out from day zero after CAR-T cell infusion. The affected person didn’t have hypotension, hypoxia, or severe biochemical change or abnormality, however had fever on day 9. Though grade 1 cytokine-release syndrome (CRS) was identified, it was efficiently handled with ibuprofen. Anti-CD7 CAR transgene copy numbers in peripheral blood have been decided by qPCR, which confirmed efficient enlargement initially, then dropped shortly, and endured at a low stage. Though skilled cytopenia from days 14 to 21, the affected person achieved remission on day 17. After full remission, the affected person acquired hematopoietic stem cell transplantation (HSCT) and has recovered effectively to thisdate. Total, this report advised that 4SCAR7 might be a protected and efficient technique for the therapy of pediatric sufferers with high-risk T-cell malignancies.
 EBAG9 siRNA |
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| 20-abx901624 | Abbexa |
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EBAG9 siRNA |
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| 20-abx914879 | Abbexa |
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EBAG9 siRNA |
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| 20-abx914880 | Abbexa |
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 EBAG9 Antibody |
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| ABD6703 | Lifescience Market | 100 ug | EUR 438 |
 HEK-293T Telomerase Over-Expressing Cell Pellet |
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| abx069991-1Pellet | Abbexa | 1 Pellet | EUR 398 |
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 EBAG9 Blocking Peptide |
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| 33R-2039 | Fitzgerald | 100 ug | EUR 180 |
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Description: A synthetic peptide for use as a blocking control in assays to test for specificity of EBAG9 antibody, catalog no. 70R-9916 |
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 Human EBAG9 Antibody |
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| 32605-05111 | AssayPro | 150 ug | EUR 261 |
EBAG9 Blocking Peptide |
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| DF6703-BP | Affbiotech | 1mg | EUR 195 |
 EBAG9 Conjugated Antibody |
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| C38323 | SAB | 100ul | EUR 397 |
 EBAG9 cloning plasmid |
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| CSB-CL007355HU1-10ug | Cusabio | 10ug | EUR 233 |
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Description: A cloning plasmid for the EBAG9 gene. |
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EBAG9 cloning plasmid |
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| CSB-CL007355HU2-10ug | Cusabio | 10ug | EUR 233 |
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Description: A cloning plasmid for the EBAG9 gene. |
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 EBAG9 Rabbit pAb |
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| A8385-100ul | Abclonal | 100 ul | EUR 308 |
EBAG9 Rabbit pAb |
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| A8385-200ul | Abclonal | 200 ul | EUR 459 |
EBAG9 Rabbit pAb |
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| A8385-20ul | Abclonal | 20 ul | Ask for price |
EBAG9 Rabbit pAb |
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| A8385-50ul | Abclonal | 50 ul | Ask for price |
EBAG9 Rabbit pAb |
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| A1935-100ul | Abclonal | 100 ul | EUR 308 |
EBAG9 Rabbit pAb |
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| A1935-200ul | Abclonal | 200 ul | EUR 459 |
EBAG9 Rabbit pAb |
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| A1935-20ul | Abclonal | 20 ul | EUR 183 |
EBAG9 Rabbit pAb |
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| A1935-50ul | Abclonal | 50 ul | EUR 223 |
) Anti-EBAG9 (4A10) |
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| YF-MA16699 | Abfrontier | 100 ug | EUR 363 |
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Description: Mouse monoclonal to EBAG9 |
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 Anti-EBAG9 antibody |
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| STJ110683 | St John's Laboratory | 100 µl | EUR 277 |
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Description: This gene was identified as an estrogen-responsive gene. Regulation of transcription by estrogen is mediated by estrogen receptor, which binds to the estrogen-responsive element found in the 5'-flanking region of this gene. The encoded protein is a tumor-associated antigen that is expressed at high frequency in a variety of cancers. Alternate splicing results in multiple transcript variants. A pseudogene of this gene has been defined on chromosome 10. |
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 Anti-EBAG9 Antibody |
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| PB9553 | BosterBio | 100ug/vial | EUR 334 |
Anti-EBAG9 antibody |
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| STJ23462 | St John's Laboratory | 100 µl | EUR 277 |
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Description: This gene was identified as an estrogen-responsive gene. Regulation of transcription by estrogen is mediated by estrogen receptor, which binds to the estrogen-responsive element found in the 5'-flanking region of this gene. The encoded protein is a tumor-associated antigen that is expressed at high frequency in a variety of cancers. Alternate splicing results in multiple transcript variants. A pseudogene of this gene has been defined on chromosome 10. |
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 pCMV-SPORT6-EBAG9 |
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| PVT13493 | Lifescience Market | 2 ug | EUR 391 |
 Chymase reagent |
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| 30C-CP1129 | Fitzgerald | 5 units | EUR 2185 |
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Description: Purified native Human Chymase reagent |
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 Traut's Reagent |
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| 2330-1000 | Biovision | EUR 349 | |
Traut's Reagent |
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| 2330-500 | Biovision | EUR 207 | |
 MTS Reagent |
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| 2808-1000 | Biovision | EUR 990 | |
MTS Reagent |
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| 2808-250 | Biovision | EUR 365 | |
 MTT Reagent |
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| 2809-1G | Biovision | EUR 180 | |
MTT Reagent |
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| 2809-5G | Biovision | EUR 544 | |
 BOP reagent |
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| 5-02141 | CHI Scientific | 25g | Ask for price |
BOP reagent |
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| 5-02142 | CHI Scientific | 100g | Ask for price |
 Bluing Reagent |
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| BRT030 | ScyTek Laboratories | 30 ml | EUR 60 |
Bluing Reagent |
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| BRT125 | ScyTek Laboratories | 125 ml | EUR 63 |
Bluing Reagent |
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| BRT3800 | ScyTek Laboratories | 1 Gal. | EUR 184 |
Bluing Reagent |
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| BRT500 | ScyTek Laboratories | 500 ml | EUR 76 |
Bluing Reagent |
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| BRT999 | ScyTek Laboratories | 1000 ml | EUR 88 |
BOP reagent |
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| A7015-100000 | ApexBio | 100 g | EUR 200 |
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Description: A peptide coupling reagent. Can be used in the preparation of phenyl esters of amino acids which have been shown to be valuable as blocked derivatives of amino acids in the field of peptide synthesis. |
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BOP reagent |
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| A7015-25000 | ApexBio | 25 g | EUR 113 |
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Description: A peptide coupling reagent. Can be used in the preparation of phenyl esters of amino acids which have been shown to be valuable as blocked derivatives of amino acids in the field of peptide synthesis. |
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 Beaucage reagent |
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| HY-100951 | MedChemExpress | 10mM/1mL | EUR 126 |
 Bradford reagent |
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| BDE641 | Bio Basic | 100ml | EUR 61.01 |
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) Polyclonal EBAG9 Antibody (Center) |
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| APR04492G | Leading Biology | 0.1ml | EUR 484 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human EBAG9 (Center). This antibody is tested and proven to work in the following applications: |
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) EBAG9 protein (His tag) |
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| 80R-1093 | Fitzgerald | 100 ug | EUR 224 |
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Description: Purified recombinant Human EBAG9 protein |
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 Rat EBAG9 shRNA Plasmid |
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| 20-abx989010 | Abbexa |
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 Mouse EBAG9 shRNA Plasmid |
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| 20-abx974569 | Abbexa |
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Polyclonal EBAG9 Antibody (Center) |
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| APR06938G | Leading Biology | 0.1ml | EUR 484 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human EBAG9 (Center). This antibody is tested and proven to work in the following applications: |
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 Human EBAG9 shRNA Plasmid |
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| 20-abx956064 | Abbexa |
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 Anti-EBAG9 / RCAS1 antibody |
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| STJ70947 | St John's Laboratory | 100 µg | EUR 359 |
) EBAG9 Recombinant Protein (Human) |
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| RP010096 | ABM | 100 ug | Ask for price |
EBAG9 Recombinant Protein (Human) |
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| RP010099 | ABM | 100 ug | Ask for price |
) EBAG9 Recombinant Protein (Rat) |
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| RP198968 | ABM | 100 ug | Ask for price |
) EBAG9 Recombinant Protein (Mouse) |
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| RP130676 | ABM | 100 ug | Ask for price |
 Arabidopsis Thaliana Lysate |
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| 30R-AA023 | Fitzgerald | 150 ug | EUR 156 |
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Description: Arabidopsis Thaliana Plant Lysate |
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 CMV Cell Lysate |
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| 35-1867 | Fitzgerald | 1 mg | EUR 1835 |
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Description: CMV enriched cell lysate |
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 HSV1 Cell Lysate |
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| 35-1872 | Fitzgerald | 1 ml | EUR 394 |
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Description: HSV1 enriched cell lysate |
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 HSV2 Cell Lysate |
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| 35-1873 | Fitzgerald | 1 ml | EUR 394 |
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Description: HSV2 enriched cell lysate |
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 JM109-lysate Antibody |
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| abx234439-100ug | Abbexa | 100 ug | EUR 551 |
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 BL21-lysate Antibody |
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| abx230905-100ug | Abbexa | 100 ug | EUR 551 |
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 DH5a-lysate Antibody |
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| abx232362-100ug | Abbexa | 100 ug | EUR 551 |
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 Green Algae Lysate |
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| PABL-1306 | Alpha Diagnostics | 50 ug | EUR 164 |
) BSA (Reagent Grade) |
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| 30-AB79 | Fitzgerald | 1 kg | EUR 1552 |
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Description: Reagent Grade Bovine Serum Albumin (99% pure) |
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BSA (Reagent Grade) |
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| 30-AB81 | Fitzgerald | 200 grams | EUR 476 |
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Description: Reagent Grade Sulphydryl Blocked BSA (99% pure) |
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 Griess Reagent Kit |
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| 30100 | Biotium | 1KIT | EUR 149 |
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Description: Minimum order quantity: 1 unit of 1KIT |
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 BODIPY-Acetylene Reagent |
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| 2594-1 | Biovision | EUR 207 | |
BODIPY-Acetylene Reagent |
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| 2594-5 | Biovision | EUR 675 | |
) Biotin reagent (HRP) |
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| 65C-CE0202 | Fitzgerald | 5 mg | EUR 244 |
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Description: HRP conjugated biotin labelling reagent |
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 Convoy? Transfection Reagent |
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| 1110-1ml | ACTGene | EUR 341 | |
 Bradford Dye Reagent |
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| 0209R | AthenaES | 100 ml | EUR 131 |
 HAMA blocking reagent |
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| 85R-1001 | Fitzgerald | 1 gram | EUR 1974 |
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Description: HAMA Blocking Reagent for use in immunoassays such as ELISA |
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HAMA blocking reagent |
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| 85R-1001P | Fitzgerald | 1 gram | EUR 2190 |
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Description: HAMA Blocking Reagent for use in immunoassays such as ELISA |
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HAMA blocking reagent |
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| 85R-1003 | Fitzgerald | 1 gram | EUR 1974 |
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Description: HAMA Blocking Reagent for use in immunoassays such as Rapid Tests |
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HAMA blocking reagent |
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| 85R-1014 | Fitzgerald | 50 mg | EUR 192 |
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Description: HAMA blocking reagent for use in assays specific for clinical false positive samples |
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HAMA blocking reagent |
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| 85R-1025 | Fitzgerald | 50 mg | EUR 192 |
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Description: HAMA blocking reagent for use in immunoassays |
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HAMA blocking reagent |
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| 85R-1026 | Fitzgerald | 50 mg | EUR 192 |
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Description: HAMA blocking reagent for use in immunoassays |
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 Girard's reagent T |
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| 20-abx184099 | Abbexa |
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 EL Transfection Reagent |
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| 20-abx098880 | Abbexa |
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 Mycoplasma Prevention Reagent |
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| 20-abx098886 | Abbexa |
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) Alcohol, Reagent (70%) |
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| EAS500 | ScyTek Laboratories | 500 ml | EUR 79 |
Alcohol, Reagent (70%) |
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| EAS999 | ScyTek Laboratories | 1000 ml | EUR 101 |
 BCA Reagent, 16ML |
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| C144-16ML | Arbor Assays | 16ML | EUR 163 |
 Biolipidure-1002-Reagent |
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| Biolipidure-1002-10 | Cusabio | 10mL | EUR 196 |
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Description: The Biolipidure-1002-Reagent is a synthetic amphoteric polymer that can be substituted for BSA in tubidimetric immunoassays. Biolipidure-1002 is an excellent blocker and also enhances assay sensitivity. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
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Biolipidure-1002-Reagent |
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| Biolipidure-1002-100 | Biolipidure | 100mL | EUR 1223 |
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Description: The Biolipidure-1002-Reagent is a synthetic amphoteric polymer that can be substituted for BSA in tubidimetric immunoassays. Biolipidure-1002 is an excellent blocker and also enhances assay sensitivity. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
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 Biolipidure-103-Reagent |
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| Biolipidure-103-10 | Biolipidure | 10mL | EUR 196 |
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Description: The Biolipidure-103-Reagent is a synthetic amphoteric polymer that can be substituted for BSA. It has been shown to enhance signals in rapid tests, western blots, and other similar immunochromatographic assays. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
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Biolipidure-103-Reagent |
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| Biolipidure-103-100 | Biolipidure | 100mL | EUR 1223 |
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Description: The Biolipidure-103-Reagent is a synthetic amphoteric polymer that can be substituted for BSA. It has been shown to enhance signals in rapid tests, western blots, and other similar immunochromatographic assays. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
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 Biolipidure-1201-Reagent |
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| Biolipidure-1201-10 | Biolipidure | 10mL | EUR 196 |
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Description: The Biolipidure-1201 Reagent is a synthetic amphoteric polymer that can be substituted for BSA. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
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Biolipidure-1201-Reagent |
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| Biolipidure-1201-100 | Biolipidure | 100mL | EUR 1223 |
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Description: The Biolipidure-1201 Reagent is a synthetic amphoteric polymer that can be substituted for BSA. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
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 Biolipidure-1301-Reagent |
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| Biolipidure-1301-10 | Biolipidure | 10mL | EUR 196 |
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Description: The Biolipidure-1301 Reagent is a synthetic amphoteric polymer that can be substituted for BSA. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
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Biolipidure-1301-Reagent |
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| Biolipidure-1301-100 | Biolipidure | 100mL | EUR 1223 |
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Description: The Biolipidure-1301 Reagent is a synthetic amphoteric polymer that can be substituted for BSA. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
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 Biolipidure-203-Reagent |
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| Biolipidure-203-10 | Biolipidure | 10mL | EUR 196 |
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Description: The Biolipidure-203 Reagent is a synthetic amphoteric polymer that can be substituted for BSA. Biolipidure-203 has been shown to enhance signal strength by improving signal-to-noise in ELISAs, EIAs, and related immunoassays. It also functions as an effective blocker and stabilizer in these assays. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
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Biolipidure-203-Reagent |
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| Biolipidure-203-100 | Biolipidure | 100mL | EUR 1223 |
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Description: The Biolipidure-203 Reagent is a synthetic amphoteric polymer that can be substituted for BSA. Biolipidure-203 has been shown to enhance signal strength by improving signal-to-noise in ELISAs, EIAs, and related immunoassays. It also functions as an effective blocker and stabilizer in these assays. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
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 Biolipidure-206-Reagent |
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| Biolipidure-206-10 | Biolipidure | 10mL | EUR 196 |
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Description: The Biolipidure-206 Reagent is a synthetic amphoteric polymer that can be substituted for BSA. Biolipidure-206 enhances signal strength, functions as an effective blocker, and stabilizes proteins and antibodies in ELISAs, EIAs, and related immunoassays. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
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Biolipidure-206-Reagent |
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| Biolipidure-206-100 | Biolipidure | 100mL | EUR 1223 |
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Description: The Biolipidure-206 Reagent is a synthetic amphoteric polymer that can be substituted for BSA. Biolipidure-206 enhances signal strength, functions as an effective blocker, and stabilizes proteins and antibodies in ELISAs, EIAs, and related immunoassays. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
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 Biolipidure-405-Reagent |
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| Biolipidure-405-10 | Biolipidure | 10mL | EUR 196 |
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Description: The Biolipidure-405 Reagent is a synthetic anionic polymer that can be used to enhance immunochromatographic assays. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
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Biolipidure-405-Reagent |
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| Biolipidure-405-100 | Biolipidure | 100mL | EUR 1223 |
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Description: The Biolipidure-405 Reagent is a synthetic anionic polymer that can be used to enhance immunochromatographic assays. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
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Affected person-Reported Outcomes for Most cancers Sufferers with Hematological Malignancies Present process Chimeric Antigen Receptor T Cell Remedy: A Systematic Assessment
Databases have been searched to establish research revealed over the previous 10 years that addressed the utility of patient-reported outcomes (PROs) in sufferers receiving chimeric antigen receptor (CAR) T cell remedy in sufferers with hematological malignancies. Amongst 280 data, three articles masking 206 sufferers have been eligible. The info have been prospectively collected at a number of time factors. The compliance charges have been 70% to 94%. There was an inverse relationship between fatigue and social perform amongst adults. The standard of life (QoL) enchancment and skill to finish PROs have been linked to illness standing. About 40% of adults reported no less than some cognitive difficulties, with a detrimental influence on psychological and bodily well being standing. In adults, probably the most generally reported cognitive impairment was reminiscence difficulties.
Melancholy was related to cognitive difficulties. Youthful adults have been at greater threat of long-term poor psychological well being, anxiousness, and despair. For pediatric and adolescent sufferers, emotional dysfunction improves over time. QoL standing improved over time; but, extreme cytokine launch syndrome and neurotoxicity precipitated delayed enchancment. Info concerning whether or not the PROs have been built-in into medical data and scientific tips is missing. Using PROs in sufferers on CAR T cell remedy appears possible and informative. Research using bigger pattern sizes and utilizing validated PRO instruments at completely different time factors stay unmet wants.