Investigation of product derived lymphoma following infusion of piggyBac modified CD19 chimeric antigen receptor T-cells
We carried out a Part I scientific trial of donor derived CD19-specific chimeric antigen receptor T-cells (CAR T-cells) for B-cell malignancy that relapsed or endured after matched associated allogeneic hemopoietic stem cell transplant. To beat the associated fee and transgene capability limitations of conventional viral vectors, CAR T-cells have been produced utilizing the piggyBac transposon system of genetic modification. Following CAR T-cell infusion, one affected person developed a progressively enlarging retroperitoneal tumor on account of a CAR expressing CD4+ T-cell lymphoma. Screening of different sufferers led to the detection of a second CAR T-cell tumor in thoracic para-aortic lymph nodes in an asymptomatic affected person.
Evaluation of the primary lymphoma confirmed a excessive transgene copy quantity, however no insertion into typical oncogenes. There have been additionally structural modifications comparable to altered genomic copy quantity and level mutations unrelated to the insertion websites. Transcriptome evaluation confirmed transgene promoter pushed upregulation of transcription of surrounding areas regardless of insulator sequences surrounding the transgene. Nevertheless, marked international modifications in transcription predominantly correlated with gene copy quantity slightly than insertion websites.
In each sufferers, the CAR T-cell derived lymphoma progressed and one affected person died. We describe the primary two instances of malignant lymphoma derived from CAR gene modified T-cells. Though CAR T-cells have an enviable report of security to this point, our outcomes emphasize the necessity for warning and common comply with up of CAR T recipients, particularly when novel strategies of gene switch are used to create genetically modified immune therapies. The trial was registered at www.anzctr.org.au as ACTRN12617001579381.
Reminiscence stem T cells modified with a redesigned CD30-chimeric antigen receptor present an enhanced antitumor impact in Hodgkin lymphoma
Goals: Adoptive cell remedy (ACT) with mature T cells modified with a chimeric antigen receptor has demonstrated improved consequence for B-cell malignancies. Nevertheless, its utility for others comparable to Hodgkin lymphoma stays a scientific problem. CD30 antigen, expressed in Hodgkin lymphoma cells, is absent in most wholesome tissues, representing a perfect goal of ACT for this illness. Regardless of that, efficacy of CD30-chimeric antigen receptor (CAR) T cells for Hodgkin lymphoma stays modest. Right here, now we have developed and examined a novel CD30-CAR T to enhance efficacy of CD30-CAR remedy, utilizing a concentrating on epitope inside the non-cleavable a part of CD30 receptor, and reminiscence stem T cells (TSCM) to enhance engraftment, persistence and antitumor exercise.
Strategies: TSCM-like cultures have been generated and expanded ex vivo and transduced at day 1 or 2 with a lentiviral vector encoding the CD30-CAR. Therapeutic in vivo experiments have been carried out utilizing NSG mice injected with L540 (sc) or L428 (iv) and handled with CD30-CAR T cells when the tumor was established.
Outcomes: CD30-CAR TSCM-like cells generated and expanded ex vivo, regardless of CD30 expression and fratricide killing of CD30+ CAR T cells, weren’t impaired by soluble CD30 and utterly eradicated Hodgkin lymphoma in vivo, displaying excessive persistence and long-lasting immunity. As well as, extremely enriched CD30-CAR TSCM-like merchandise confer a survival benefit in vivo, in distinction to extra differentiated CAR T cells, with greater tumor infiltration and enhanced antitumor impact.
Conclusion: This research helps using a refined CD30-CAR T cells with extremely enriched TSCM-like merchandise to enhance scientific efficacy of CAR T for Hodgkin lymphoma.
Chimeric antigen receptor T cells concentrating on CD7 in a toddler with high-risk T-cell acute lymphoblastic leukemia
Efficient systemic therapies for relapsed or refractory T-cell acute lymphoblastic leukemia (T-ALL) are restricted. Current scientific utility of chimeric antigen receptor (CAR) immunotherapy has demonstrated profitable management of B-cell malignancies by CAR-T cells; nonetheless, designing CARs for T-ALL stays a problem. CD7 overexpression in T-cell malignancies could also be a horny goal for immunotherapy in T-ALL. This research aimed to explain the protected and efficient use of autologous CD7-CAR T cells (4SCAR7) for the therapy of T-ALL with induction failure in an 11-year-old affected person. Based mostly on The Chinese language Youngsters’s Most cancers Group-ALL (CCCG-ALL) research protocol, minimal residual illness (MRD) by move cytometry (FC) evaluation was detected on days 19 and 46 of remission induction.
On the finish of remission-induction chemotherapy, the affected person achieved morphologic full remission, although with MRD 16.13% and RT-PCR of KMT2A-MLLT1 fusion constructive, which indicated induction failure. The cerebrospinal fluid (CSF) was damaging for blasts at identified. CAR-T remedy and allogeneic transplant have been really helpful as the subsequent therapy choices. CD3+ lymphocytes have been collected from the affected person 18 days after the high-dose MTX chemotherapy by means of leukapheresis. The 4SCAR7 CD7-targeting CAR-T cells have been generated thereafter.
The affected person acquired lymphodepleting chemotherapy previous to 4SCAR7 infusion. Oral administration of itraconazole and sulfamethoxazole was carried out from day zero after CAR-T cell infusion. The affected person didn’t have hypotension, hypoxia, or severe biochemical change or abnormality, however had fever on day 9. Though grade 1 cytokine-release syndrome (CRS) was identified, it was efficiently handled with ibuprofen. Anti-CD7 CAR transgene copy numbers in peripheral blood have been decided by qPCR, which confirmed efficient enlargement initially, then dropped shortly, and endured at a low stage. Though skilled cytopenia from days 14 to 21, the affected person achieved remission on day 17. After full remission, the affected person acquired hematopoietic stem cell transplantation (HSCT) and has recovered effectively to thisdate. Total, this report advised that 4SCAR7 might be a protected and efficient technique for the therapy of pediatric sufferers with high-risk T-cell malignancies.

GFP expression Adenovirus, in vivo ready |
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AVP011-PBS | GenTarget | 1x1011 IFU/ml x 50ul | EUR 852 |
Description: pre-made GFP expression adenovirus, provided in PBS solution. |
YFP expression Adenovirus, in vivo ready |
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AVP012-PBS | GenTarget | 1x1011 IFU/ml x 50ul | EUR 852 |
Description: pre-made YFP expression adenovirus, provided in PBS solution. |
BFP expression Adenovirus, in vivo ready |
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AVP017-PBS | GenTarget | 1x1011 IFU/ml x 50ul | EUR 852 |
Description: pre-made BFP expression adenovirus, provided in PBS solution. |
Blunt E1 Expression Kit |
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abx098098-10rxns | Abbexa | 10 rxns | EUR 560.4 |
Blunt E2 Expression Kit |
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abx098873-10rxns | Abbexa | 10 rxns | EUR 560.4 |
RFP expression Adenovirus |
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AVP001 | GenTarget | 1x109 IFU/ml x 200ul | EUR 418.8 |
Description: pre-made RFP expression adenovirus, provided in DMEM medium. |
CFP expression Adenovirus |
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AVP002 | GenTarget | 1x109 IFU/ml x 200ul | EUR 418.8 |
Description: pre-made CFP expression adenovirus, provided in DMEM medium. |
GFP expression Adenovirus |
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AVP011 | GenTarget | 1x109 IFU/ml x 200ul | EUR 418.8 |
Description: pre-made GFP expression adenovirus, provided in DMEM medium. |
YFP expression Adenovirus |
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AVP012 | GenTarget | 1x109 IFU/ml x 200ul | EUR 418.8 |
Description: pre-made YFP expression adenovirus, provided in DMEM medium. |
BFP expression Adenovirus |
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AVP017 | GenTarget | 1x109 IFU/ml x 200ul | EUR 418.8 |
Description: pre-made BFP expression adenovirus, provided in DMEM medium. |
Rac1 Expression Vector Set |
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STA-454 | Cell Biolabs | 1 kit | EUR 1765.2 |
Description: Rac1 Expression Vector Set contains 3 vectors: Rac wild type, T17N dominant negative mutant, and G12V constitutively active mutant. |
RhoA Expression Vector Set |
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STA-456 | Cell Biolabs | 1 kit | EUR 1765.2 |
Description: RhoA Expression Vector Set contains 3 vectors: RhoA wild type, T19N dominant negative mutant, and G14V constitutively active mutant. |
h OCT4 expression Adenovirus, in vivo ready |
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AVP013-PBS | GenTarget | 1x1011 IFU/ml x 50ul | EUR 852 |
Description: pre-made adenovirus express human stem cell factor, OCT4, provided in PBS solution for in vivo application. |
h SOX2 expression Adenovirus, in vivo ready |
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AVP014-PBS | GenTarget | 1x1011 IFU/ml x 50ul | EUR 852 |
Description: pre-made adenovirus express human stem cell factor, SOX2, provided in PBS solution for in vivo application. |
Cdc42 Expression Vector Set |
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STA-455 | Cell Biolabs | 1 kit | EUR 1765.2 |
Description: Cdc42 Expression Vector Set contains 3 vectors: Cdc42 wild type, T17N dominant negative mutant, and Q61L constitutively active mutant. |
h LIN28 expression Adenovirus, in vivo ready |
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AVP015-PBS | GenTarget | 1x1011 IFU/ml x 50ul | EUR 852 |
Description: pre-made adenovirus express human stem cell factor, LIN28, provided in PBS solution for in vivo application. |
h ADA Expression Lentivirus |
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LVP923 | GenTarget | 1x107 IFU/ml x 200ul | EUR 541.2 |
Description: Pre-made over-expression lentivirus for human target: ADA, containing a RFP-Blasticidin dual selection marker. |
h IL6 Expression Lentivirus |
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LVP1144 | GenTarget | 1x107 IFU/ml x 200ul | EUR 541.2 |
Description: Pre-made over-expression lentivirus for human target: HDAC1, containing a RFP-Blasticidin dual selection marker. |
h CD4 Expression Lentivirus |
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LVP1152 | GenTarget | 1x107 IFU/ml x 200ul | EUR 541.2 |
Description: Pre-made over-expression lentivirus for human target: CD4, containing a RFP-Blasticidin dual selection marker. |
h IL4 Expression Lentivirus |
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LVP1161 | GenTarget | 1x107 IFU/ml x 200ul | EUR 541.2 |
Description: Pre-made over-expression lentivirus for human target: IL4, containing a RFP-Blasticidin dual selection marker. |
h IL7 Expression Lentivirus |
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LVP1162 | GenTarget | 1x107 IFU/ml x 200ul | EUR 541.2 |
Description: Pre-made over-expression lentivirus for human target: IL7, containing a RFP-Blasticidin dual selection marker. |
h KDR Expression Lentivirus |
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LVP1176 | GenTarget | 1x107 IFU/ml x 200ul | EUR 541.2 |
Description: Pre-made over-expression lentivirus for human target: KDR, containing a RFP-Blasticidin dual selection marker. |
h FAS Expression Lentivirus |
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LVP1181 | GenTarget | 1x107 IFU/ml x 200ul | EUR 541.2 |
Description: Pre-made over-expression lentivirus for human target: FAS, containing a RFP-Blasticidin dual selection marker. |
pAAV-MCS Expression Vector |
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VPK-410 | Cell Biolabs | 10 µg | EUR 776.4 |
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV. |
h OCT4 expression Adenovirus |
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AVP013 | GenTarget | 1x109 IFU/ml x 200ul | EUR 418.8 |
Description: pre-made adenovirus express human stem cell factor, OCT4, provided in DMEM medium. |
h SOX2 expression Adenovirus |
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AVP014 | GenTarget | 1x109 IFU/ml x 50ul | EUR 418.8 |
Description: pre-made adenovirus express human stem cell factor, SOX2, provided in DMEM medium. |
h CDR1 Expression Lentivirus |
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LVP917 | GenTarget | 1x107 IFU/ml x 200ul | EUR 541.2 |
Description: Pre-made over-expression lentivirus for human target: CDR1, containing a RFP-Blasticidin dual selection marker. |
h CD47 Expression Lentivirus |
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LVP918 | GenTarget | 1x107 IFU/ml x 200ul | EUR 541.2 |
Description: Pre-made over-expression lentivirus for human target: CD47, containing a RFP-Blasticidin dual selection marker. |
h ESR1 Expression Lentivirus |
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LVP924 | GenTarget | 1x107 IFU/ml x 200ul | EUR 541.2 |
Description: Pre-made over-expression lentivirus for human target: ESR1, containing a RFP-Blasticidin dual selection marker. |
r Syn1 Expression Lentivirus |
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LVP925 | GenTarget | 1x107 IFU/ml x 200ul | EUR 541.2 |
Description: Pre-made over-expression lentivirus for rat target: Syn1, containing a RFP-Blasticidin dual selection marker. |
h NME4 Expression Lentivirus |
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LVP932 | GenTarget | 1x107 IFU/ml x 200ul | EUR 541.2 |
Description: Pre-made over-expression lentivirus for human target: NME4, containing a RFP-Blasticidin dual selection marker. |
h EGFR Expression Lentivirus |
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LVP1142 | GenTarget | 1x107 IFU/ml x 200ul | EUR 541.2 |
Description: Pre-made over-expression lentivirus for human target: EGFR, containing a RFP-Blasticidin dual selection marker. |
h NRP1 Expression Lentivirus |
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LVP1145 | GenTarget | 1x107 IFU/ml x 200ul | EUR 541.2 |
Description: Pre-made over-expression lentivirus for human target: HDAC1, containing a RFP-Blasticidin dual selection marker. |
h MUC1 Expression Lentivirus |
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LVP1148 | GenTarget | 1x107 IFU/ml x 200ul | EUR 541.2 |
Description: Pre-made over-expression lentivirus for human target: HDAC1, containing a RFP-Blasticidin dual selection marker. |
h CD8a Expression Lentivirus |
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LVP1151 | GenTarget | 1x107 IFU/ml x 200ul | EUR 541.2 |
Description: Pre-made over-expression lentivirus for human target: CD8a, containing a RFP-Blasticidin dual selection marker. |
h PIGF Expression Lentivirus |
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LVP1154 | GenTarget | 1x107 IFU/ml x 200ul | EUR 541.2 |
Description: Pre-made over-expression lentivirus for human target: PIGF, containing a RFP-Blasticidin dual selection marker. |
h BRAF Expression Lentivirus |
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LVP1156 | GenTarget | 1x107 IFU/ml x 200ul | EUR 541.2 |
Description: Pre-made over-expression lentivirus for human target: BRAF, containing a RFP-Blasticidin dual selection marker. |
h ROS1 Expression Lentivirus |
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LVP1158 | GenTarget | 1x107 IFU/ml x 200ul | EUR 541.2 |
Description: Pre-made over-expression lentivirus for human target: ROS1, containing a RFP-Blasticidin dual selection marker. |
h CD3E Expression Lentivirus |
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LVP1163 | GenTarget | 1x107 IFU/ml x 200ul | EUR 541.2 |
Description: Pre-made over-expression lentivirus for human target: IL7, containing a RFP-Blasticidin dual selection marker. |
h KLK3 Expression Lentivirus |
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LVP1171 | GenTarget | 1x107 IFU/ml x 200ul | EUR 541.2 |
Description: Pre-made over-expression lentivirus for human target: KLK3, containing a RFP-Blasticidin dual selection marker. |
h NEK6 Expression Lentivirus |
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LVP1182 | GenTarget | 1x107 IFU/ml x 200ul | EUR 541.2 |
Description: Pre-made over-expression lentivirus for human target: NEK6, containing a RFP-Blasticidin dual selection marker. |
h NEK7 Expression Lentivirus |
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LVP1183 | GenTarget | 1x107 IFU/ml x 200ul | EUR 541.2 |
Description: Pre-made over-expression lentivirus for human target: NEK7, containing a RFP-Blasticidin dual selection marker. |
H-Ras Expression Vector Set |
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STA-457 | Cell Biolabs | 1 kit | EUR 1765.2 |
Description: H-Ras Expression Vector Set contains 3 vectors: H-Ras wild type, T19N dominant negative mutant, and G12V constitutively active mutant. |
h LIN28 expression Adenovirus |
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AVP015 | GenTarget | 1x109 IFU/ml x 200ul | EUR 418.8 |
Description: pre-made adenovirus express human stem cell factor, LIN28, provided in DMEM medium. |
h GPNMB Expression Lentivirus |
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LVP921 | GenTarget | 1x107 IFU/ml x 200ul | EUR 541.2 |
Description: Pre-made over-expression lentivirus for human target: GPNMB, containing a RFP-Blasticidin dual selection marker. |
h DECR1 Expression Lentivirus |
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LVP927 | GenTarget | 1x107 IFU/ml x 200ul | EUR 541.2 |
Description: Pre-made over-expression lentivirus for human target: DECR1, containing a RFP-Blasticidin dual selection marker. |
h DECR2 Expression Lentivirus |
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LVP928 | GenTarget | 1x107 IFU/ml x 200ul | EUR 541.2 |
Description: Pre-made over-expression lentivirus for human target: DECR2, containing a RFP-Blasticidin dual selection marker. |
h NMRK1 Expression Lentivirus |
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LVP930 | GenTarget | 1x107 IFU/ml x 200ul | EUR 541.2 |
Description: Pre-made over-expression lentivirus for human target: NMRK1, containing a RFP-Blasticidin dual selection marker. |
h PITX3 Expression Lentivirus |
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LVP931 | GenTarget | 1x107 IFU/ml x 200ul | EUR 541.2 |
Description: Pre-made over-expression lentivirus for human target: PITX3, containing a RFP-Blasticidin dual selection marker. |
h HDAC1 Expression Lentivirus |
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LVP1143 | GenTarget | 1x107 IFU/ml x 200ul | EUR 541.2 |
Description: Pre-made over-expression lentivirus for human target: HDAC1, containing a RFP-Blasticidin dual selection marker. |
h VEGFB Expression Lentivirus |
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LVP1146 | GenTarget | 1x107 IFU/ml x 200ul | EUR 541.2 |
Description: Pre-made over-expression lentivirus for human target: HDAC1, containing a RFP-Blasticidin dual selection marker. |
h PARP1 Expression Lentivirus |
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LVP1149 | GenTarget | 1x107 IFU/ml x 200ul | EUR 541.2 |
Description: Pre-made over-expression lentivirus for human target: HDAC1, containing a RFP-Blasticidin dual selection marker. |
h IL3RA Expression Lentivirus |
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LVP1150 | GenTarget | 1x107 IFU/ml x 200ul | EUR 541.2 |
Description: Pre-made over-expression lentivirus for human target: IL3RA, containing a RFP-Blasticidin dual selection marker. |
h PTCH1 Expression Lentivirus |
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LVP1155 | GenTarget | 1x107 IFU/ml x 200ul | EUR 541.2 |
Description: Pre-made over-expression lentivirus for human target: PTCH1, containing a RFP-Blasticidin dual selection marker. |
h SART3 Expression Lentivirus |
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LVP1159 | GenTarget | 1x107 IFU/ml x 200ul | EUR 541.2 |
Description: Pre-made over-expression lentivirus for human target: SART3, containing a RFP-Blasticidin dual selection marker. |
h TIGIT Expression Lentivirus |
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LVP1160 | GenTarget | 1x107 IFU/ml x 200ul | EUR 541.2 |
Description: Pre-made over-expression lentivirus for human target: TIGIT, containing a RFP-Blasticidin dual selection marker. |
h EDNRA Expression Lentivirus |
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LVP1165 | GenTarget | 1x107 IFU/ml x 200ul | EUR 541.2 |
Description: Pre-made over-expression lentivirus for human target: EDNRA containing a RFP-Blasticidin dual selection marker. |
h Foxp3 Expression Lentivirus |
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LVP1172 | GenTarget | 1x107 IFU/ml x 200ul | EUR 541.2 |
Description: Pre-made over-expression lentivirus for human target: Foxp3, containing a RFP-Blasticidin dual selection marker. |
m RIKEN Expression Lentivirus |
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LVP1174 | GenTarget | 1x107 IFU/ml x 200ul | EUR 541.2 |
Description: Pre-made over-expression lentivirus for mouse target: RIKEN, containing a RFP-Blasticidin dual selection marker. |
h APLP2 Expression Lentivirus |
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LVP1177 | GenTarget | 1x107 IFU/ml x 200ul | EUR 541.2 |
Description: Pre-made over-expression lentivirus for human target: APLP2, containing a RFP-Blasticidin dual selection marker. |
h PCSK9 Expression Lentivirus |
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LVP1178 | GenTarget | 1x107 IFU/ml x 200ul | EUR 541.2 |
Description: Pre-made over-expression lentivirus for human target: PCSK9, containing a RFP-Blasticidin dual selection marker. |
h NTSR1 Expression Lentivirus |
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LVP1179 | GenTarget | 1x107 IFU/ml x 200ul | EUR 541.2 |
Description: Pre-made over-expression lentivirus for human target: NTSR1, containing a RFP-Blasticidin dual selection marker. |
pscAAV-MCS Expression Vector |
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VPK-430 | Cell Biolabs | 10 µg | EUR 776.4 |
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV. |
m CTAGA1 Expression Lentivirus |
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LVP916 | GenTarget | 1x107 IFU/ml x 200ul | EUR 541.2 |
Description: Pre-made over-expression lentivirus for mouse target: CTAGA1, containing a RFP-Blasticidin dual selection marker. |
h SKIV2L Expression Lentivirus |
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LVP919 | GenTarget | 1x107 IFU/ml x 200ul | EUR 541.2 |
Description: Pre-made over-expression lentivirus for human target: SKIV2L, containing a RFP-Blasticidin dual selection marker. |
h SKIV2L Expression Lentivirus |
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LVP919-GP | GenTarget | 1x107 IFU/ml x 200ul | EUR 541.2 |
Description: Pre-made over-expression lentivirus for human target: SKIV2L, containing a GFP-Puromycin dual selection marker. |
h TP5313 Expression Lentivirus |
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LVP926 | GenTarget | 1x107 IFU/ml x 200ul | EUR 541.2 |
Description: Pre-made over-expression lentivirus for human target: TP5313, containing a RFP-Blasticidin dual selection marker. |
h ELOVL5 Expression Lentivirus |
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LVP929 | GenTarget | 1x107 IFU/ml x 200ul | EUR 541.2 |
Description: Pre-made over-expression lentivirus for human target: ELOVL5, containing a RFP-Blasticidin dual selection marker. |
h MAGEA3 Expression Lentivirus |
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LVP1147 | GenTarget | 1x107 IFU/ml x 200ul | EUR 541.2 |
Description: Pre-made over-expression lentivirus for human target: HDAC1, containing a RFP-Blasticidin dual selection marker. |
h NANOS3 Expression Lentivirus |
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LVP1164 | GenTarget | 1x107 IFU/ml x 200ul | EUR 541.2 |
Description: Pre-made over-expression lentivirus for human target: NANOS3, containing a RFP-Blasticidin dual selection marker. |
h IL1RL1 Expression Lentivirus |
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LVP1168 | GenTarget | 1x107 IFU/ml x 200ul | EUR 541.2 |
Description: Pre-made over-expression lentivirus for human target: IL1RL1, containing a RFP-Blasticidin dual selection marker. |
h CTAG1B Expression Lentivirus |
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LVP1169 | GenTarget | 1x107 IFU/ml x 200ul | EUR 541.2 |
Description: Pre-made over-expression lentivirus for human target: CTAG1B, containing a RFP-Blasticidin dual selection marker. |
h MAGEA3 Expression Lentivirus |
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LVP1170 | GenTarget | 1x107 IFU/ml x 200ul | EUR 541.2 |
Description: Pre-made over-expression lentivirus for human target: MAGEA3, containing a RFP-Blasticidin dual selection marker. |
h COX7A1 Expression Lentivirus |
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LVP1173 | GenTarget | 1x107 IFU/ml x 200ul | EUR 541.2 |
Description: Pre-made over-expression lentivirus for human target: COX7A1, containing a RFP-Blasticidin dual selection marker. |
h BCL11B Expression Lentivirus |
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LVP1180 | GenTarget | 1x107 IFU/ml x 200ul | EUR 541.2 |
Description: Pre-made over-expression lentivirus for human target: BCL11B, containing a RFP-Blasticidin dual selection marker. |
pUC57-sgRNA expression vector |
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PVT18003 | Lifescience Market | 2 ug | EUR 309.6 |
BRAF, His-Tag (High Expression) |
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41237 | BPS Bioscience | 10 µg | EUR 375 |
Description: Human BRAF, also known as B-RAF1 and RAFB1, with 18 mutations to improve expression; S446A, S447A, I543A, I544S, I551K, Q562R, L588N, K630S, F667E, Y672S, A688R, L706S, Q709R, S713E, L716E, S720E, P722S, K723G. GenBank Accession #NM_004333, a.a. 444-723 with N-terminal His-tag, expressed in E. coli cells. MW = 33 kDa. |
Human Receptor-binding cancer antigen expressed on SiSo cells (EBAG9) |
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1-CSB-EP007355HU | Cusabio |
|
|
Description: Recombinant Human Receptor-binding cancer antigen expressed on SiSo cells(EBAG9),partial expressed in E.coli |
ANPRA/Aequorin Expression vector |
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60641 | BPS Bioscience | 20 µg | EUR 900 |
Description: The ANPRA/Aequorin expression vector is designed to co-express human atrial natriuretic peptide receptor A (ANPRA, also called natriuretic peptide receptor A/guanylate cyclase A) and jellyfish (Aequorea victoria) Aequorin in mammalian cells. |
h CEACAM5 Expression Lentivirus |
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LVP1153 | GenTarget | 1x107 IFU/ml x 200ul | EUR 541.2 |
Description: Pre-made over-expression lentivirus for human target: CEACAM5, containing a RFP-Blasticidin dual selection marker. |
h TNFSF11 Expression Lentivirus |
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LVP1157 | GenTarget | 1x107 IFU/ml x 200ul | EUR 541.2 |
Description: Pre-made over-expression lentivirus for human target: TNFSF11, containing a RFP-Blasticidin dual selection marker. |
h TNFRSF1B Expression Lentivirus |
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LVP922 | GenTarget | 1x107 IFU/ml x 200ul | EUR 541.2 |
Description: Pre-made over-expression lentivirus for human target: TNFRSF1B, containing a RFP-Blasticidin dual selection marker. |
h KIAA1324 Expression Lentivirus |
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LVP1175 | GenTarget | 1x107 IFU/ml x 200ul | EUR 541.2 |
Description: Pre-made over-expression lentivirus for human target: KIAA1324, containing a RFP-Blasticidin dual selection marker. |
GFP-Rac1 Expression Vector Set |
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STA-450 | Cell Biolabs | 1 kit | EUR 1939.2 |
Description: GFP-Rac1 Expression Vector Set contains 3 vectors: Rac wild type, T17N dominant negative mutant, and Q61L constitutively active mutant. Each vector also contains a GFP reporter sequence. |
GFP-RhoA Expression Vector Set |
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STA-452 | Cell Biolabs | 1 kit | EUR 1939.2 |
Description: GFP-RhoA Expression Vector Set contains 3 vectors: RhoA wild type, T19N dominant negative mutant, and Q63L constitutively active mutant. Each vector also contains a GFP reporter sequence. |
Receptor-Binding Cancer Antigen Expressed On SiSo Cells (EBAG9) Antibody |
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20-abx112338 | Abbexa |
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Receptor-Binding Cancer Antigen Expressed On SiSo Cells (EBAG9) Antibody |
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abx031290-400ul | Abbexa | 400 ul | EUR 627.6 |
Receptor-Binding Cancer Antigen Expressed On SiSo Cells (EBAG9) Antibody |
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abx031290-80l | Abbexa | 80 µl | EUR 343.2 |
Receptor-Binding Cancer Antigen Expressed On SiSo Cells (EBAG9) Antibody |
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abx037482-100ug | Abbexa | 100 ug | EUR 469.2 |
Receptor-Binding Cancer Antigen Expressed On SiSo Cells (EBAG9) Antibody |
|||
abx037831-100ug | Abbexa | 100 ug | EUR 469.2 |
Receptor-Binding Cancer Antigen Expressed On SiSo Cells (EBAG9) Antibody |
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20-abx123391 | Abbexa |
|
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Receptor-Binding Cancer Antigen Expressed On SiSo Cells (EBAG9) Antibody |
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abx430429-200ul | Abbexa | 200 ul | EUR 460.8 |
Receptor-Binding Cancer Antigen Expressed On SiSo Cells (EBAG9) Antibody |
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20-abx001581 | Abbexa |
|
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h KIAA0319L Expression Lentivirus |
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LVP920 | GenTarget | 1x107 IFU/ml x 200ul | EUR 541.2 |
Description: Pre-made over-expression lentivirus for human target: KIAA0319L, containing a RFP-Blasticidin dual selection marker. |
GFP-Cdc42 Expression Vector Set |
|||
STA-451 | Cell Biolabs | 1 kit | EUR 1939.2 |
Description: GFP-Cdc42 Expression Vector Set contains 3 vectors: Cdc42 wild type, T17N dominant negative mutant, and Q61L constitutively active mutant. Each vector also contains a GFP reporter sequence. |
Active Rac1 Expression Vector Set |
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STA-458 | Cell Biolabs | 1 kit | EUR 1765.2 |
Description: Active Rac1 Expression Vector Set contains 3 vectors expressing different constitutively active mutants of Rac1: Q61L, Q61L/F37A, and Q61L/Y40C. |
PhiC31 Integrase Expression Plasmid |
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FC200PA-1 | SBI | 10ug | EUR 496 |
pCMV-GFP-LC3 Expression Vector |
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CBA-401 | Cell Biolabs | 100 µL | EUR 1243.2 |
Description: Expression vector contains a fusion of GFP and LC3. A separate GFP control vector is also included. |
h KRAS (G12D) Expression Lentivirus |
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LVP1166 | GenTarget | 1x107 IFU/ml x 200ul | EUR 541.2 |
Description: Pre-made over-expression lentivirus for a mutant (G12D) of human target: KRAS containing a RFP-Blasticidin dual selection marker. |
Tet-on circRNA Expression Vector |
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PVT14643 | Lifescience Market | 2 ug | EUR 843.6 |
h TP53 (R175H) Expression Lentivirus |
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LVP1167 | GenTarget | 1x107 IFU/ml x 200ul | EUR 541.2 |
Description: Pre-made over-expression lentivirus for a mutant R175H) of human target: TP53, containing a RFP-Blasticidin dual selection marker. |
pAAV-IRES-Neo Expression Vector |
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VPK-416 | Cell Biolabs | 10 µg | EUR 776.4 |
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV. |
pAAV-IRES-GFP Expression Vector |
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VPK-418 | Cell Biolabs | 10 µg | EUR 776.4 |
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV. |
pAAV-IRES-Bsd Expression Vector |
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VPK-419 | Cell Biolabs | 10 µg | EUR 776.4 |
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV. |
PinPoint Integrase Expression Plasmid |
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PIN200A-1 | SBI | 10ug | EUR 688 |
pAAV-IRES-Puro Expression Vector |
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VPK-415 | Cell Biolabs | 10 µg | EUR 776.4 |
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV. |
CEACAM5 CHO Cell Line (Low Expression) |
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78704-L | BPS Bioscience | 2 vials | EUR 7150 |
Description: Recombinant clonal CHO cell line stably expressing full-length human CEACAM5 (NM_004363). Surface expression of CEACAM5 was confirmed by flow cytometry. The stable clonal cell line was selected for different levels of CEACAM5 expression (High, Medium, and Low) compared to the parental CHO-K1 cell line. |
Active H-Ras Expression Vector Set |
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STA-459 | Cell Biolabs | 1 kit | EUR 1765.2 |
Description: Active H-Ras Expression Vector Set contains 3 vectors expressing different constitutively active mutants of H-Ras: V12, V12S35, and V12C40. |
B7-H3 CHO Cell Line (Low Expression) |
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78541-L | BPS Bioscience | 2 vials | EUR 7150 |
Description: B7 homolog 3 protein (B7-H3) CHO Cell line is a recombinant clonal stable CHO cell line expressing full length human B7-H3 protein, also known as cluster of differentiation 276 (CD276, B7H3; B7RP-2; 4Ig-B7-H3). Surface expression of B7-H3 was confirmed by flow cytometry. Each stable clonal cell line was selected for High, Medium, or Low levels of B7-H3 expression to mimic different B7-H3 expression levels in various cancer types. |
pAAV-IRES-Hygro Expression Vector |
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VPK-417 | Cell Biolabs | 10 µg | EUR 776.4 |
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV. |
Affected person-Reported Outcomes for Most cancers Sufferers with Hematological Malignancies Present process Chimeric Antigen Receptor T Cell Remedy: A Systematic Assessment
Databases have been searched to establish research revealed over the previous 10 years that addressed the utility of patient-reported outcomes (PROs) in sufferers receiving chimeric antigen receptor (CAR) T cell remedy in sufferers with hematological malignancies. Amongst 280 data, three articles masking 206 sufferers have been eligible. The info have been prospectively collected at a number of time factors. The compliance charges have been 70% to 94%. There was an inverse relationship between fatigue and social perform amongst adults. The standard of life (QoL) enchancment and skill to finish PROs have been linked to illness standing. About 40% of adults reported no less than some cognitive difficulties, with a detrimental influence on psychological and bodily well being standing. In adults, probably the most generally reported cognitive impairment was reminiscence difficulties.
Melancholy was related to cognitive difficulties. Youthful adults have been at greater threat of long-term poor psychological well being, anxiousness, and despair. For pediatric and adolescent sufferers, emotional dysfunction improves over time. QoL standing improved over time; but, extreme cytokine launch syndrome and neurotoxicity precipitated delayed enchancment. Info concerning whether or not the PROs have been built-in into medical data and scientific tips is missing. Using PROs in sufferers on CAR T cell remedy appears possible and informative. Research using bigger pattern sizes and utilizing validated PRO instruments at completely different time factors stay unmet wants.