Reliably isolate mononuclear cells from peripheral blood, cord blood, or bone marrow with Lymphoprep™, a cost-effective alternative to Ficoll-Paque™. Use this density gradient medium for rapid, simple, and reliable cell isolation from most blood samples obtained from normal individuals and patients. You can substitute Lymphoprep™ for Ficoll-Paque™ without changing your existing protocols and achieve similar cell purity and recovery rates. This medium is fully compatible with SepMate™ and RosetteSep™. Formulated with sodium diatrizoate (9.1% w/v) and polysaccharide (5.7% w/v), Lymphoprep™ has a density of 1.077 g/mL.
In 1968, Dr Arne Bø yum reported a simple and effective method for the isolation of mononuclear cells from human blood. For more than 35 years, a commercial outlet known as Lymphoprep™ has been widely used for the isolation of these cells. Mononuclear cells (monocytes and lymphocytes) have a lower buoyant density than erythrocytes and polymorphonuclear (PMN) leukocytes (granulocytes). The vast majority of mononuclear cells have densities less than 1.077 g/ml.
Therefore, these cells can be isolated by centrifugation in an isoosmotic medium with a density of 1.077 µg/mL, allowing erythrocytes and PMNs to sediment in the medium while retaining mononuclear cells at the sample/medium interface. The described method is fast, simple and reliable and gives excellent results with blood samples from normal individuals and patients. For maximum performance, it is important that the blood sample is diluted 1:1 with normal saline before applying it to the gradient. Contamination of erythrocytes in the mononuclear cell suspension is usually between 3-10% of the total number of cells.
Some immature PMNs can bind to lymphocytes during intense immunosuppressive therapy. When heparinized blood is used, it is essential to remove most of the platelets, to avoid inhibition in the cytotoxicity assay. Lymphoprep™ can be used for the preparation of suspensions of pure lymphocytes for tissue typing, antilymphocyte sera, and immunological research. Thorsby and Brattelie used this technique with slight modifications in the preparation of pure lymphocyte suspensions for cytotoxicity tests and lymphocyte cultures.