ultrasonic-scientific

receptor binding affinity towards the wild type rather than anchor-modified antigen

A framework for extremely multiplexed dextramer mapping and prediction of T cell receptor sequences to antigen specificity

T cell receptor (TCR) antigen-specific recognition is important for the adaptive immune system. Nevertheless, constructing a TCR-antigen interplay map has been difficult because of the staggering range of TCRs and antigens. Accordingly, extremely multiplexed dextramer-TCR binding assays have been just lately developed, however the utility of the following giant datasets is restricted by the shortage of sturdy computational strategies for normalization and interpretation.

Right here, we current a computational framework comprising a novel technique, ICON (Integrative COntext-specific Normalization), for figuring out dependable TCR-pMHC (peptide-major histocompatibility complicated) interactions and a neural network-based classifier TCRAI that outperforms different state-of-the-art strategies for TCR-antigen specificity prediction. We additional demonstrated that by combining ICON and TCRAI, we’re capable of uncover novel subgroups of TCRs that bind to a given pMHC through totally different mechanisms. Our framework facilitates the identification and understanding of TCR-antigen-specific interactions for fundamental immunological analysis and medical immune monitoring.

 

Quantifying persistence within the T-cell signaling community utilizing an optically controllable antigen receptor

T cells discriminate between wholesome and contaminated cells with outstanding sensitivity when mounting an immune response, which is hypothesized to rely on T cells combining stimuli from a number of antigen-presenting cell interactions right into a stronger response. To quantify the capability for T cells to perform this, we now have developed an antigen receptor that’s optically tunable inside cell conjugates, offering management over the length, and depth of intracellular T-cell signaling.

We observe restricted persistence inside the T-cell intracellular community on disruption of receptor enter, with indicators dissipating solely in ~15 min, and straight present sustained proximal receptor signaling is required to keep up gene transcription. T cells thus primarily accumulate the outputs of gene expression slightly than combine discrete intracellular indicators. Engineering optical management in a clinically related chimeric antigen receptor (CAR), we present that this restricted sign persistence will be exploited to extend CAR-T cell activation threefold utilizing pulsatile stimulation. Our outcomes are prone to apply extra typically to the signaling dynamics of different mobile networks.

Tumor rejection properties of gp100 209-specific T cells correlate with T cell receptor binding affinity in direction of the wild sort slightly than anchor-modified antigen

Though there are exceptions and outliers, T cell useful responses typically correlate with the affinity of a TCR for a peptide/MHC complicated. In a single just lately described outlier case, essentially the most promising medical candidate in a sequence of TCRs particular for the gp100209 melanoma antigen certain with the weakest resolution affinity and produced the least quantity of cytokine in vitro. Hypotheses for this outlier conduct included uncommon cytokine expression patterns arising from an atypical TCR binding geometry. Learning this occasion in additional element, we discovered right here that outlier conduct is attributable to not uncommon cytokine patterns or TCR binding, however the usage of a place 2 anchor-modified peptide variant in in vitro experiments as an alternative of the wild sort antigen that’s current in vivo.

Though the anchor-modified variant has been extensively utilized in fundamental and medical immunology as a surrogate for the wild sort peptide, prior work has proven that TCRs can clearly distinguish between the 2. We present that when this differential recognition is accounted for, the useful properties of gp100209-specific TCRs observe with their affinity in direction of the peptide/MHC complicated. Past demonstrating the correlates with T cell operate for a clinically related TCR, our outcomes present necessary concerns for choice of TCRs for immunotherapy and the usage of modified peptides in immunology.

ultrasonic-scientific
ultrasonic-scientific

Recombinant Human RCAS1 / EBAG9 protein (His tag)

PDEH100304-20ug 20ug
EUR 68
Description: Human

Recombinant Human RCAS1/ EBAG9 Protein, His-SUMO, E.coli-1mg

QP5959-ec-1mg 1mg
EUR 1958.4

Recombinant Human RCAS1/ EBAG9 Protein, His-SUMO, E.coli-10ug

QP5959-ec-10ug 10ug
EUR 240

Recombinant Human RCAS1/ EBAG9 Protein, His-SUMO, E.coli-50ug

QP5959-ec-50ug 50ug
EUR 315.6

Recombinant Human RCAS1/ EBAG9 Protein, His-SUMO, E.coli-100ug

QP5959-ec-100ug 100ug
EUR 489.6

Recombinant Human RCAS1/ EBAG9 Protein, His-SUMO, E.coli-200ug

QP5959-ec-200ug 200ug
EUR 760.8

Recombinant Human RCAS1/ EBAG9 Protein, His-SUMO, E.coli-500ug

QP5959-ec-500ug 500ug
EUR 1272

Recombinant Human Receptor-binding cancer antigen expressed on SiSo cells(EBAG9),partial

AP70252 1mg
EUR 1978

Recombinant Human Receptor-binding cancer antigen expressed on SiSo cells (EBAG9), partial

CSB-EP007355HU 1056 mg Ask for price

Recombinant CD155 Protein (Asp 28-Asn 343)

VAng-1181Lsx-100g 100 µg
EUR 1215.6
Description: Rhesus macaque CD155 is expressed in HEK 293 cells. (Uniprot ID: Q0MSE6-1)

Recombinant CD155 Protein (Asp 28-Asn 343)

VAng-1181Lsx-1mg 1 mg
EUR 7682.4
Description: Rhesus macaque CD155 is expressed in HEK 293 cells. (Uniprot ID: Q0MSE6-1)

Recombinant ALCAM Protein (Trp 28-Ala 526) [Fc]

VAng-0714Lsx-1mg 1 mg
EUR 3294
Description: Human ALCAM, Fc tag, is expressed in HEK 293 cells. (Uniprot ID: NP_001618)

Recombinant ALCAM Protein (Trp 28-Ala 526) [Fc]

VAng-0714Lsx-200g 200 µg
EUR 951.6
Description: Human ALCAM, Fc tag, is expressed in HEK 293 cells. (Uniprot ID: NP_001618)

1110001A16Rik Recombinant Protein (Mouse)

RP109673 100 ug Ask for price

1110001A16Rik Recombinant Protein (Mouse)

RP109676 100 ug Ask for price

1110005A03Rik Recombinant Protein (Mouse)

RP109697 100 ug Ask for price

1110032A03Rik Recombinant Protein (Mouse)

RP109781 100 ug Ask for price

1110032A04Rik Recombinant Protein (Mouse)

RP109784 100 ug Ask for price

1110032A04Rik Recombinant Protein (Mouse)

RP109787 100 ug Ask for price

1110034A24Rik Recombinant Protein (Mouse)

RP109793 100 ug Ask for price

1520402A15Rik Recombinant Protein (Mouse)

RP109955 100 ug Ask for price

1700006A11Rik Recombinant Protein (Mouse)

RP110042 100 ug Ask for price

1700008A04Rik Recombinant Protein (Mouse)

RP110066 100 ug Ask for price

1700011A15Rik Recombinant Protein (Mouse)

RP110105 100 ug Ask for price

1700012A03Rik Recombinant Protein (Mouse)

RP110129 100 ug Ask for price

1700012A16Rik Recombinant Protein (Mouse)

RP110132 100 ug Ask for price

1700019A02Rik Recombinant Protein (Mouse)

RP110213 100 ug Ask for price

1700020A23Rik Recombinant Protein (Mouse)

RP110249 100 ug Ask for price

1700020A23Rik Recombinant Protein (Mouse)

RP110252 100 ug Ask for price

1700023A16Rik Recombinant Protein (Mouse)

RP110291 100 ug Ask for price

1700027A23Rik Recombinant Protein (Mouse)

RP110336 100 ug Ask for price

1700027A23Rik Recombinant Protein (Mouse)

RP110339 100 ug Ask for price

1810008A18Rik Recombinant Protein (Mouse)

RP110603 100 ug Ask for price

1810009A15Rik Recombinant Protein (Mouse)

RP110606 100 ug Ask for price

2010305A19Rik Recombinant Protein (Mouse)

RP110798 100 ug Ask for price

2300009A05Rik Recombinant Protein (Mouse)

RP110906 100 ug Ask for price

2310001A20Rik Recombinant Protein (Mouse)

RP110909 100 ug Ask for price

2310022A10Rik Recombinant Protein (Mouse)

RP110996 100 ug Ask for price

2310022A10Rik Recombinant Protein (Mouse)

RP110999 100 ug Ask for price

2310046A06Rik Recombinant Protein (Mouse)

RP111062 100 ug Ask for price

2410004A20Rik Recombinant Protein (Mouse)

RP111134 100 ug Ask for price

2610528A11Rik Recombinant Protein (Mouse)

RP111308 100 ug Ask for price

2810403A07Rik Recombinant Protein (Mouse)

RP111407 100 ug Ask for price

2810408A11Rik Recombinant Protein (Mouse)

RP111416 100 ug Ask for price

2900026A02Rik Recombinant Protein (Mouse)

RP111464 100 ug Ask for price

2900053A13Rik Recombinant Protein (Mouse)

RP111467 100 ug Ask for price

2900064A13Rik Recombinant Protein (Mouse)

RP111470 100 ug Ask for price

3110003A17Rik Recombinant Protein (Mouse)

RP111491 100 ug Ask for price

3830417A13Rik Recombinant Protein (Mouse)

RP111569 100 ug Ask for price

4921523A10Rik Recombinant Protein (Mouse)

RP111704 100 ug Ask for price

4930404A10Rik Recombinant Protein (Mouse)

RP111758 100 ug Ask for price

4930427A07Rik Recombinant Protein (Mouse)

RP111794 100 ug Ask for price

4930430A15Rik Recombinant Protein (Mouse)

RP111803 100 ug Ask for price

4930431A04Rik Recombinant Protein (Mouse)

RP111812 100 ug Ask for price

4930444A02Rik Recombinant Protein (Mouse)

RP111839 100 ug Ask for price

4930465A12Rik Recombinant Protein (Mouse)

RP111884 100 ug Ask for price

4930468A15Rik Recombinant Protein (Mouse)

RP111890 100 ug Ask for price

4930505A04Rik Recombinant Protein (Mouse)

RP111926 100 ug Ask for price

4930521A18Rik Recombinant Protein (Mouse)

RP111953 100 ug Ask for price

4930557A04Rik Recombinant Protein (Mouse)

RP112037 100 ug Ask for price

4931408A02Rik Recombinant Protein (Mouse)

RP112133 100 ug Ask for price

4931408A02Rik Recombinant Protein (Mouse)

RP112136 100 ug Ask for price

4933417A18Rik Recombinant Protein (Mouse)

RP112316 100 ug Ask for price

6030405A18Rik Recombinant Protein (Mouse)

RP112559 100 ug Ask for price

6330403A02Rik Recombinant Protein (Mouse)

RP112592 100 ug Ask for price

6330545A04Rik Recombinant Protein (Mouse)

RP112628 100 ug Ask for price

8430410A17Rik Recombinant Protein (Mouse)

RP112721 100 ug Ask for price

9030625A04Rik Recombinant Protein (Mouse)

RP112763 100 ug Ask for price

9430007A20Rik Recombinant Protein (Mouse)

RP112820 100 ug Ask for price

A130010J15Rik Recombinant Protein (Mouse)

RP112922 100 ug Ask for price

A130010J15Rik Recombinant Protein (Mouse)

RP112925 100 ug Ask for price

A130010J15Rik Recombinant Protein (Mouse)

RP112928 100 ug Ask for price

A130022J15Rik Recombinant Protein (Mouse)

RP112931 100 ug Ask for price

A230050P20Rik Recombinant Protein (Mouse)

RP112943 100 ug Ask for price

A230051G13Rik Recombinant Protein (Mouse)

RP112946 100 ug Ask for price

A230065H16Rik Recombinant Protein (Mouse)

RP112949 100 ug Ask for price

A330049M08Rik Recombinant Protein (Mouse)

RP112961 100 ug Ask for price

A330049M08Rik Recombinant Protein (Mouse)

RP112964 100 ug Ask for price

A330049M08Rik Recombinant Protein (Mouse)

RP112967 100 ug Ask for price

A330049M08Rik Recombinant Protein (Mouse)

RP112970 100 ug Ask for price

A330049M08Rik Recombinant Protein (Mouse)

RP112973 100 ug Ask for price

A330049M08Rik Recombinant Protein (Mouse)

RP112976 100 ug Ask for price

A330050F15Rik Recombinant Protein (Mouse)

RP112979 100 ug Ask for price

A430005L14Rik Recombinant Protein (Mouse)

RP112988 100 ug Ask for price

A430005L14Rik Recombinant Protein (Mouse)

RP112991 100 ug Ask for price

A430033K04Rik Recombinant Protein (Mouse)

RP112994 100 ug Ask for price

A430078G23Rik Recombinant Protein (Mouse)

RP112997 100 ug Ask for price

A430084P05Rik Recombinant Protein (Mouse)

RP113000 100 ug Ask for price

A430089I19Rik Recombinant Protein (Mouse)

RP113003 100 ug Ask for price

A430105I19Rik Recombinant Protein (Mouse)

RP113006 100 ug Ask for price

A430107P09Rik Recombinant Protein (Mouse)

RP113012 100 ug Ask for price

A530016L24Rik Recombinant Protein (Mouse)

RP113027 100 ug Ask for price

A530032D15Rik Recombinant Protein (Mouse)

RP113030 100 ug Ask for price

A530064D06Rik Recombinant Protein (Mouse)

RP113036 100 ug Ask for price

A530064D06Rik Recombinant Protein (Mouse)

RP113039 100 ug Ask for price

A530098C11Rik Recombinant Protein (Mouse)

RP113042 100 ug Ask for price

A530099J19Rik Recombinant Protein (Mouse)

RP113045 100 ug Ask for price

A630001G21Rik Recombinant Protein (Mouse)

RP113048 100 ug Ask for price

A630033H20Rik Recombinant Protein (Mouse)

RP113057 100 ug Ask for price

A630033H20Rik Recombinant Protein (Mouse)

RP113060 100 ug Ask for price

A630033H20Rik Recombinant Protein (Mouse)

RP113063 100 ug Ask for price

A630055G03Rik Recombinant Protein (Mouse)

RP113066 100 ug Ask for price

A630073D07Rik Recombinant Protein (Mouse)

RP113069 100 ug Ask for price

A630095E13Rik Recombinant Protein (Mouse)

RP113075 100 ug Ask for price

A630095N17Rik Recombinant Protein (Mouse)

RP113078 100 ug Ask for price

A630095N17Rik Recombinant Protein (Mouse)

RP113081 100 ug Ask for price

A730008H23Rik Recombinant Protein (Mouse)

RP113084 100 ug Ask for price

A730011L01Rik Recombinant Protein (Mouse)

RP113087 100 ug Ask for price

A730011L01Rik Recombinant Protein (Mouse)

RP113090 100 ug Ask for price

A730017C20Rik Recombinant Protein (Mouse)

RP113093 100 ug Ask for price

A730017C20Rik Recombinant Protein (Mouse)

RP113096 100 ug Ask for price

A730037C10Rik Recombinant Protein (Mouse)

RP113099 100 ug Ask for price

A830007P12Rik Recombinant Protein (Mouse)

RP113102 100 ug Ask for price

A830018L16Rik Recombinant Protein (Mouse)

RP113111 100 ug Ask for price

A830018L16Rik Recombinant Protein (Mouse)

RP113114 100 ug Ask for price

A830018L16Rik Recombinant Protein (Mouse)

RP113117 100 ug Ask for price

A830018L16Rik Recombinant Protein (Mouse)

RP113120 100 ug Ask for price

A830080D01Rik Recombinant Protein (Mouse)

RP113123 100 ug Ask for price

A830093I24Rik Recombinant Protein (Mouse)

RP113126 100 ug Ask for price

A930001N09Rik Recombinant Protein (Mouse)

RP113129 100 ug Ask for price

A930005I04Rik Recombinant Protein (Mouse)

RP113132 100 ug Ask for price

A930018P22Rik Recombinant Protein (Mouse)

RP113138 100 ug Ask for price

A930038C07Rik Recombinant Protein (Mouse)

RP113141 100 ug Ask for price

Recombinant ALCAM Protein (Trp 28-Ala 526) [His]

VAng-0715Lsx-1mg 1 mg
EUR 3096
Description: Human ALCAM, His tag, is expressed in HEK 293 cells. (Uniprot ID: Q13740-1)

Recombinant ALCAM Protein (Trp 28-Ala 526) [His]

VAng-0715Lsx-200g 200 µg
EUR 885.6
Description: Human ALCAM, His tag, is expressed in HEK 293 cells. (Uniprot ID: Q13740-1)

EBAG9 protein (His tag)

80R-1093 100 ug
EUR 219
Description: Purified recombinant Human EBAG9 protein

Recombinant Erythropoietin Protein (Ala 28-Arg 193)

VAng-1516Lsx-20g 20 µg
EUR 712.8
Description: Human Erythropoietin (EPO) is expressed in HEK 293 cells. (Uniprot ID: AAH93628.1)

Recombinant Erythropoietin Protein (Ala 28-Arg 193)

VAng-1516Lsx-50g 50 µg
EUR 1215.6
Description: Human Erythropoietin (EPO) is expressed in HEK 293 cells. (Uniprot ID: AAH93628.1)

Rat IL-13 (109aa) Recombinant Protein

R20-031 10 µg
EUR 196.35
Description: IL-13 is an immunoregulatory cytokine produced primarily by activated Th2 cells, and also by mast cells and NK cells. Targeted deletion of IL-13 in mice resulted in impaired Th2 cell development and indicated an important role for IL-13 in the expulsion of gastrointestinal parasites. IL-13 exerts anti-inflammatory effects on monocytes and macrophages and it inhibits the expression of inflammatory cytokines such as IL-1β, TNF-α, IL-6 and IL-8. IL-13 has also been shown to enhance B cell proliferation and to induce isotype switching resulting in increased production of IgE. Blocking of IL-13 activity inhibits the pathophysiology of asthma. Human and murine IL-13 is cross-species reactive. Recombinant rat IL-13 is an 11.9 kDa protein consisting of 109 amino acid residues.

Rat IL-13 (109aa) Recombinant Protein

R20-031S 2 µg
EUR 92.4
Description: IL-13 is an immunoregulatory cytokine produced primarily by activated Th2 cells, and also by mast cells and NK cells. Targeted deletion of IL-13 in mice resulted in impaired Th2 cell development and indicated an important role for IL-13 in the expulsion of gastrointestinal parasites. IL-13 exerts anti-inflammatory effects on monocytes and macrophages and it inhibits the expression of inflammatory cytokines such as IL-1β, TNF-α, IL-6 and IL-8. IL-13 has also been shown to enhance B cell proliferation and to induce isotype switching resulting in increased production of IgE. Blocking of IL-13 activity inhibits the pathophysiology of asthma. Human and murine IL-13 is cross-species reactive. Recombinant rat IL-13 is an 11.9 kDa protein consisting of 109 amino acid residues.

Rat IL-13 (113aa) Recombinant Protein

R20-049 10 µg
EUR 196.35
Description: IL-13 is an immunoregulatory cytokine produced primarily by activated Th2 cells, and also by mast cells and NK cells. Targeted deletion of IL-13 in mice resulted in impaired Th2 cell development and indicated an important role for IL-13 in the expulsion of gastrointestinal parasites. IL-13 exerts anti-inflammatory effects on monocytes and macrophages and it inhibits the expression of inflammatory cytokines such as IL-1β, TNF-α, IL-6 and IL-8. IL-13 has also been shown to enhance B cell proliferation and to induce isotype switching resulting in increased production of IgE. Blocking of IL-13 activity inhibits the pathophysiology of asthma. Human and murine IL-13 is cross-species reactive. Recombinant rat IL-13 is a 12.3 kDa protein consisting of 113 amino acid residues.

Rat IL-13 (113aa) Recombinant Protein

R20-049S 2 µg
EUR 92.4
Description: IL-13 is an immunoregulatory cytokine produced primarily by activated Th2 cells, and also by mast cells and NK cells. Targeted deletion of IL-13 in mice resulted in impaired Th2 cell development and indicated an important role for IL-13 in the expulsion of gastrointestinal parasites. IL-13 exerts anti-inflammatory effects on monocytes and macrophages and it inhibits the expression of inflammatory cytokines such as IL-1β, TNF-α, IL-6 and IL-8. IL-13 has also been shown to enhance B cell proliferation and to induce isotype switching resulting in increased production of IgE. Blocking of IL-13 activity inhibits the pathophysiology of asthma. Human and murine IL-13 is cross-species reactive. Recombinant rat IL-13 is a 12.3 kDa protein consisting of 113 amino acid residues.

OPPA02054-10UG - EBAG9 Protein

OPPA02054-10UG 10ug
EUR 70

Human IL-8 (77aa) Recombinant Protein

100-047 25 µg
EUR 196.35
Description: IL-8 is a proinflammatory CXC chemokine that can signal through the CXCR1 and CXCR2 receptors. It is secreted by monocytes and endothelial cells. IL-8 chemoattracts and activates neutrophils. Recombinant human IL-8 (endothelial-derived) is an 8.9 kDa protein containing 77 amino acid residues.

Human IL-8 (77aa) Recombinant Protein

100-047S 5 µg
EUR 92.4
Description: IL-8 is a proinflammatory CXC chemokine that can signal through the CXCR1 and CXCR2 receptors. It is secreted by monocytes and endothelial cells. IL-8 chemoattracts and activates neutrophils. Recombinant human IL-8 (endothelial-derived) is an 8.9 kDa protein containing 77 amino acid residues.

Human BD-1 (36aa) Recombinant Protein

100-132 20 µg
EUR 196.35
Description: Defensins (alpha and beta) are cationic peptides with a broad spectrum of antimicrobial activity that comprise an important arm of the innate immune system. The alpha-defensins are distinguished from the beta-defensins by the pairing of their three disulfide bonds. To date, four human beta-defensins have been identified; BD-1, BD-2, BD-3 and BD-4. Beta-defensins are expressed on some leukocytes and at epithelial surfaces. In addition to their direct antimicrobial activities, they are chemoattractant towards immature dendritic cells and memory T cells. The beta-defensin proteins are expressed as the C-terminal portion of precursors and are released by proteolytic cleavage of a signal sequence and, in the case of BD-1 (36 a.a.), a propeptide region. Beta-defensins contain a six-cysteine motif that forms three intra-molecular disulfide bonds. Beta-Defensins are 3-5 kDa peptides ranging in size from 33-47 amino acid residues. Recombinant Human BD-1 is a 3.9 kDa protein containing 36 amino acid residues.

Human BD-1 (36aa) Recombinant Protein

100-132S 5 µg
EUR 92.4
Description: Defensins (alpha and beta) are cationic peptides with a broad spectrum of antimicrobial activity that comprise an important arm of the innate immune system. The alpha-defensins are distinguished from the beta-defensins by the pairing of their three disulfide bonds. To date, four human beta-defensins have been identified; BD-1, BD-2, BD-3 and BD-4. Beta-defensins are expressed on some leukocytes and at epithelial surfaces. In addition to their direct antimicrobial activities, they are chemoattractant towards immature dendritic cells and memory T cells. The beta-defensin proteins are expressed as the C-terminal portion of precursors and are released by proteolytic cleavage of a signal sequence and, in the case of BD-1 (36 a.a.), a propeptide region. Beta-defensins contain a six-cysteine motif that forms three intra-molecular disulfide bonds. Beta-Defensins are 3-5 kDa peptides ranging in size from 33-47 amino acid residues. Recombinant Human BD-1 is a 3.9 kDa protein containing 36 amino acid residues.

Human BD-1 (47aa) Recombinant Protein

100-133 20 µg
EUR 196.35
Description: Defensins (alpha and beta) are cationic peptides with a broad spectrum of antimicrobial activity that comprise an important arm of the innate immune system. The alpha-defensins are distinguished from the beta-defensins by the pairing of their three disulfide bonds. To date, four human beta-defensins have been identified; BD-1, BD-2, BD-3 and BD-4. Beta-defensins are expressed on some leukocytes and at epithelial surfaces. In addition to their direct antimicrobial activities, they are chemoattractant towards immature dendritic cells and memory T cells. The Beta-defensin proteins are expressed as the C-terminal portion of precursors and are released by proteolytic cleavage of a signal sequence and, in the case of BD-1 (36 a.a.), a propeptide region. Beta-defensins contain a six-cysteine motif that forms three intra-molecular disulfide bonds. Beta-Defensins are 3-5 kDa peptides ranging in size from 33-47 amino acid residues. Recombinant Human BD-1 is a 5.0 kDa protein containing 47 amino acid residues.

Human BD-1 (47aa) Recombinant Protein

100-133S 5 µg
EUR 92.4
Description: Defensins (alpha and beta) are cationic peptides with a broad spectrum of antimicrobial activity that comprise an important arm of the innate immune system. The alpha-defensins are distinguished from the beta-defensins by the pairing of their three disulfide bonds. To date, four human beta-defensins have been identified; BD-1, BD-2, BD-3 and BD-4. Beta-defensins are expressed on some leukocytes and at epithelial surfaces. In addition to their direct antimicrobial activities, they are chemoattractant towards immature dendritic cells and memory T cells. The Beta-defensin proteins are expressed as the C-terminal portion of precursors and are released by proteolytic cleavage of a signal sequence and, in the case of BD-1 (36 a.a.), a propeptide region. Beta-defensins contain a six-cysteine motif that forms three intra-molecular disulfide bonds. Beta-Defensins are 3-5 kDa peptides ranging in size from 33-47 amino acid residues. Recombinant Human BD-1 is a 5.0 kDa protein containing 47 amino acid residues.

Human EBAG9 Protein, His Tag

E40KMPH4470 20ug
EUR 495

S100 calcium binding protein A15A Recombinant Protein

92-713 0.05 mg
EUR 588.3
Description: S100 calcium binding protein A15A Recombinant Protein

Human EBAG9 Protein Lysate 20ug

IHUEBAG9PLLY20UG each
EUR 213
Description: Human EBAG9 Protein Lysate 20ug

GWB-P1243H-50UG - TAU, 1-383aa, Recombinant Protein

GWB-P1243H-50UG 50ug
EUR 440

Human Amphiregulin (98aa) Recombinant Protein

100-393 50 µg
EUR 196.35
Description: Amphiregulin is an EGF related growth factor that signals through the EGF/TGF-a receptor, and stimulates growth of keratinocytes, epithelial cells and some fibroblasts. Amphiregulin also inhibits the growth of certain carcinoma cell lines. Synthesized as a transmembrane protein, Amphiregulin’s extracellular domain is proteolytically processed to release the mature protein. There are 6 conserved cysteine residues, which form 3 intramolecular disulfide bonds essential for biological activity. Recombinant human Amphiregulin is a 11.3 kDa glycoprotein consisting of 98 amino acid residues.

Human Amphiregulin (98aa) Recombinant Protein

100-393S 10 µg
EUR 92.4
Description: Amphiregulin is an EGF related growth factor that signals through the EGF/TGF-a receptor, and stimulates growth of keratinocytes, epithelial cells and some fibroblasts. Amphiregulin also inhibits the growth of certain carcinoma cell lines. Synthesized as a transmembrane protein, Amphiregulin’s extracellular domain is proteolytically processed to release the mature protein. There are 6 conserved cysteine residues, which form 3 intramolecular disulfide bonds essential for biological activity. Recombinant human Amphiregulin is a 11.3 kDa glycoprotein consisting of 98 amino acid residues.

GWB-P0876E-50UG - DCPS, 1-337aa, Recombinant Protein

GWB-P0876E-50UG 50ug
EUR 440

GWB-P0950A-50UG - POMC, 27-267aa, Recombinant Protein

GWB-P0950A-50UG 50ug
EUR 440

GWB-P1115J-50UG - MYL9, 1-172aa, Recombinant Protein

GWB-P1115J-50UG 50ug
EUR 330

GWB-P1243H-250UG - TAU, 1-383aa, Recombinant Protein

GWB-P1243H-250UG 250ug
EUR 1320

GWB-P0361J-100UG - HBA2, 1-142aa, Recombinant Protein

GWB-P0361J-100UG 100ug
EUR 330

GWB-P0361J-500UG - HBA2, 1-142aa, Recombinant Protein

GWB-P0361J-500UG 500ug
EUR 990

GWB-P0872A-100UG - KARS, 63-597aa, Recombinant Protein

GWB-P0872A-100UG 100ug
EUR 330

GWB-P0872A-500UG - KARS, 63-597aa, Recombinant Protein

GWB-P0872A-500UG 500ug
EUR 990

GWB-P0876E-250UG - DCPS, 1-337aa, Recombinant Protein

GWB-P0876E-250UG 250ug
EUR 1320

GWB-P0950A-250UG - POMC, 27-267aa, Recombinant Protein

GWB-P0950A-250UG 250ug
EUR 1320

GWB-P1115J-250UG - MYL9, 1-172aa, Recombinant Protein

GWB-P1115J-250UG 250ug
EUR 990

GWB-P1588B-100UG - MNDA, 1-407aa, Recombinant Protein

GWB-P1588B-100UG 100ug
EUR 660

GWB-P1588B-500UG - MNDA, 1-407aa, Recombinant Protein

GWB-P1588B-500UG 500ug
EUR 1980

GWB-P1576C-100UG - PDIA3, 25-505aa, Recombinant Protein

GWB-P1576C-100UG 100ug
EUR 286

GWB-P1576C-500UG - PDIA3, 25-505aa, Recombinant Protein

GWB-P1576C-500UG 500ug
EUR 858

Proliferating cell nuclear antigen straight interacts with androgen receptor and enhances androgen receptor‑mediated signaling

Androgen receptor (AR) and/or its constitutively lively splicing variants (AR‑Vs), reminiscent of AR‑V7 and ARv567es, is required for prostate most cancers cell development and survival, and most cancers development. Proliferating cell nuclear antigen (PCNA) is preferentially overexpressed in all cancers and executes its capabilities by way of interplay with quite a few accomplice proteins. The goal of the current research was to research the potential position of PCNA within the regulation of AR exercise.

An equivalent consensus sequence of the PCNA‑interacting protein‑field (PIP‑field) was recognized on the N‑terminus of human, mouse and rat AR proteins. It was discovered that PCNA complexes with the total‑size AR (AR‑FL) and AR‑V7, which will be attenuated by the small molecule PIP‑field inhibitor, T2AA. PCNA additionally complexes with ARv567es and recombinant AR protein. The PCNA inhibitors, PCNA‑I1S and T2AA, inhibited AR transcriptional exercise and the expression of AR goal genes in LNCaP‑AI and 22Rv1 cells, however not in AR‑detrimental PC‑three cells. The knockdown of PCNA expression lowered dihydrotestosterone‑stimulated AR transcriptional exercise and abolished the inhibitory impact of PCNA‑I1S on AR exercise. The PCNA inhibitor, PCNA‑I1, exerted additive development inhibitory results with androgen deprivation and enzalutamide in cells expressing AR‑FL or AR‑FL/AR‑V7, however not in AR‑detrimental PC‑three cells.

Lastly, R9‑AR‑PIP, a small peptide mimicking AR PIP‑field, was discovered to bind to GFP‑PCNA at Okd of two.73 µM and inhibit the expression of AR goal genes, AR transcriptional exercise and the expansion of AR‑expressing cells. On the entire, these information strongly recommend that AR is a PCNA accomplice protein and interacts with PCNA through the PIP‑field and that focusing on the PCNA‑AR interplay might characterize an progressive and selective therapeutic technique towards prostate most cancers, notably castration‑resistant prostate cancers overexpressing constitutively lively AR‑Vs.

Novel high-affinity EGFRvIII-specific chimeric antigen receptor T cells successfully remove human glioblastoma

Targets: The rising success of Chimeric Antigen Receptor (CAR) T cell remedy in haematological malignancies is reinvigorating its software in lots of different most cancers varieties and with renewed concentrate on its software to strong tumors. We current a novel CAR towards glioblastoma, an aggressive, malignant glioma, with a dismal survival charge for which therapy choices have remained unchanged for over a decade.

Strategies: We use the human Retained Show (ReD) antibody platform (Myrio Therapeutics) to establish a novel single-chain variable fragment (scFv) that recognises epidermal development issue receptor mutant variant III (EGFRvIII), a typical and tumor-specific mutation present in glioblastoma. We use each in vitro useful assays and an in vivo orthotopic xenograft mannequin of glioblastoma to look at the operate of our novel CAR, known as GCT02, focused utilizing murine CAR T cells.

Outcomes: Our EGFRvIII-specific scFv was discovered to be of a lot increased affinity than reported comparators reverse-engineered from monoclonal antibodies. Regardless of the upper affinity, GCT02 CAR T cells kill equivalently however secrete decrease quantities of cytokine. As well as, GCT02-CAR T cells additionally mediate fast and full tumor elimination in vivo.

Conclusion: We current a novel EGFRvIII-specific CAR, with efficient antitumor capabilities each in in vitro and in a xenograft mannequin of human glioblastoma.

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